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KMID : 0361319870190010203
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Korean Journal of Medical Technologists
1987 Volume.19 No. 1 p.203 ~ p.207
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Quantitative Analysis of HBs-Ab Measured by Radioimmunoassay
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Abstract
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The detection system uses a "sandwich principle" a solid phase radioimmunoassay technique, to measure Anti-HBs levels in serum. Plastic beads coated with human hepatitis B surface Antigen(HBsAg) are supplied in the Abbott kit.
The estimated RIA unit value is determined by calculating the ratio of the reactivity of the unknown specimen to the positive control and selecting the estimated RIA unit value for this ratio from table. Calculate the ratip for the undiluted specimen and for the dilution specimen.
It is anticipated that in almost every instance either an undiluted or diluted specimen ratio was used the RIA unit.
I carried out the experiments on the changes according to the dilution facter for the positive control serum and positive patients.
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